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What do bacteria containing a desired gene also contain that makes them need a specific amino acid in a nutrient medium in order to grow?

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Excerpt from Bartolomé de Las Casas's Destruction of the Indies, 1552 :Among the gentle sheep, gifted by their Maker with the above qualities, the Spaniards entered as soon as they knew them, like wolves, tigers, and lions which had been starving for many days, and since forty years they have done nothing else; nor do they otherwise at the present day, than outrage, slay, afflict, torment, and destroy them with strange and new, and divers kinds of cruelty, never before seen, nor heard of, nor read of, of which some few will be told below: to such extremes has this gone that, whereas there were more than three million souls, whom we saw in Hispaniola, there are to-day, not two hundred of the native population left.The island of Cuba is almost as long as the distance from Valladolid to Rome; it is now almost entirely deserted. The islands of San Juan [Porto Rico], and Jamaica, very large and happy and pleasing islands, are both desolate. The Lucaya Isles lie near Hispaniola and Cuba to the north and number more than sixty, including those that are called the Giants, and other large and small Islands; the poorest of these, which is more fertile, and pleasing than the King's garden in Se
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The concept of Manifest Destiny was primarily used to justify...A) The annexation of Texas.B) The Oregon Trail migration.C) The Mexican-American War.D) Westward expansion to the Pacific Ocean.

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