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E. coli ribonuclease H1 is an enzyme that catalyzes the hydrolysis of phosphodiester bonds in RNA. Its proposed mechanism involves a “carboxylate relay.”a) Using the structures below as a guide, draw arrows that indicate how the hydrolysis reaction might be initiated.b) The pK values for all of the histidines in ribonuclease H1 were determined and include values of 7.1, 5.5, and < 5.0. Which value is most likely to correspond to His 124? Explain.c) Substituting an alanine residue for His 124 resulted in a dramatic decrease in enzyme activity. Explain.
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