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1-Le contact declenche le transfert Le pilus se rétracte et un pont cytoplasmique se forme 2-Coupure de l'un des 2 brins du plasmide au niveau de oriT 3-Seul un brin du plasmide F ou F' entre dans la receveuse, l'autre reste dans la donneuse Chacun des 2 brins d'ADN est dupliqué (neosynthetisation)Déroulement du plasmide pour libérer le brin transféré 4-La receveuse devient donneuse Dans le cas d'une donneuse F' le plasmide apporte un morceau d'ADN chromosomique
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